tReasure (tRNA Expression Analysis Software Utilizing R for Easy use) is user-friendly tool for the tRNA expression analysis of deep-sequencing experiments for small RNAs using R packages.

tReasure currently implements the following tasks:
1) Making sample list for analysis
2) Pre-processing of trimming adapter and filtering reads
3) Alignment and quantification
4) Statistical analysis for differentially expressed tRNAs gene
5) Visualization 

tReasure is a package for the R computing environment and it is assumed that you have already installed R according to your operating system. See the R project at (http://www.r-project.org). tReasure requires the gwidget2 graphical library to run and a few additional packages for the analysis of RNA-seq.

• tReasure works fine with version R4.1. After being updated to the latest R version 4.2, an error has been identified in the tReasure. As soon as possible, we will revise the code and announce it again.
• Being tested using conda as an alternative to install.
  • 2022-11-02 completion: under R4.1 using conda on Ubuntu.
  • 2022-11-02 ongoing: under R4.1 using conda on Mac

1. For window users :
   User need to install Rtools before installing tReasure.

2. For linux users :
   Users need to install libcurl4-openssl-dev and libssl-dev for devtools.

   • Example for user Ubuntu

   sudo apt-get install libcurl4-openssl-dev 
   sudo apt-get install libssl-dev
   

   Also, user need to install libgtk2.0-dev and libxml2-dev for tReasure.

   • Example for user Ubuntu

   sudo apt-get update -y
   sudo apt-get install -y libxml2-dev
   sudo apt-get install -y libgtk2.0-dev
   

3. For Mac OS users :
   Users need to install opnssl for devtools.

   • Example using brew

   brew update
   brew install openssl
   

   Users need to install XQuartz, gtk2 and cairo before installing tReasure.

   • Example using brew

   brew install cairo  
   brew install gtk+
   

It takes a few minutes to install for the first time.

 install.packages("devtools")
 library("devtools")

 devtools::install_github("jinoklee/tReasure",force = TRUE)
 library("tReasure")
 install.tReasure()

Note If the descriptioin shows as below during the installation, choose "Install GTK+"

Need GTK+? (Restart Required)
    Install GTK+
    Do not install GTK+

Note If the installation was successful, the tReasure window appears. If the window dose not appear, restart R and type as below.

tReasure::tReasure()

1. For Ubuntu :

• Install miniconda and create enviroment

  conda create --name r4.1
  conda activate r4.1
  

• Install Mamba for bioinformatics packages in conda and need to update conda.

  conda install mamba -c conda-forge -y
  conda update conda -y
  conda update -all 
  

• Install R 4.1 and required packages.

  mamba install -c conda-forge r-base=4.1.2
  mamba install -c conda-forge r-devtools
  mamba install -c conda-forge r-rgtk2
  mamba install -c conda-forge r-cairodevice
  

• Open R and type as below

  library("devtools") 
  devtools::install_github("jinoklee/tReasure",force = TRUE)
  library("tReasure")
  install.tReasure()
  

 library("tReasure")
 tReasure::tReasure()

• Download

• Click

• tReasure is automatically downloaded during analysis. If an error occurs during download, we recommend that you download the file directly, and then move it to a specific location, and unzip it.
  • Make folder (Hsapi38 or Mmusc10) on tReasure package folder. ex) [R4.1 PATH]/library/tReasure/extdata/refer/Hsapi38/
  • Move and extract the downloaded files into the folder created.
  • Homo sapiens hg38
  • Mus musculus 10

• Small RNA-seq dataset from human breast tissues (part of GSE68085)
  • Download
  • Control (Normal): SRR1982473, SRR1982474, SRR1982475
  • Test (Cancer): SRR1982580, SRR1982581, SRR1982582

• Installation and setup: Window
• Full version of analysis: Window Mac
• Results folders: Click